LOCUS       pLV-[hsa-miR-381]        9129 bp    DNA     circular      3-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7050..7910
                     /label=Amp(R)
     promoter        8111..8651
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5655..5889
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6464..6919)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5961..6091
                     /label=SV40 pA
     misc_feature    6092..6305
                     /label=SV40 ori
     misc_feature    4976..5572
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3594
                     /label=intron
     CDS             3641..4966
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3384
                     /label=hsa-mir-381 precursor
BASE COUNT     2155 a      2376 c      2482 g      2116 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca tcccgcatct 
     3121 cccaccccct cccccgccag gatgaacccc gggagaggag gccggccccg tgaatgatag 
     3181 tgaggaacct gcccagtgct attgtttggt acttaaagcg aggttgccct ttgtatattc 
     3241 ggtttattga catggaatat acaagggcaa gctctctgtg agtatcaaac cttgtcttgg 
     3301 acccagtcca cactcagcaa gggatgcggt atgtgtgtgc atgaccgtgt gtatccgttt 
     3361 tgtgtgtggc aaatcgttca cggggctagc tcgagctttt ggagtacgtc gtctttaggt 
     3421 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 
     3481 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 
     3541 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 
     3601 tgaaaactac ccctctagag tcgagctacc ggtcgccacc atggtgagca agggcgagga 
     3661 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt 
     3721 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca 
     3781 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc 
     3841 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta 
     3901 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg 
     3961 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt 
     4021 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg 
     4081 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa 
     4141 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa 
     4201 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac 
     4261 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc 
     4321 caccggcggc atggacgagc tgtacaagga cccaccggtc gccaccatga ccgagtacaa 
     4381 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 
     4441 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 
     4501 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 
     4561 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 
     4621 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 
     4681 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 
     4741 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 
     4801 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 
     4861 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc 
     4921 gaaggaccgc gcgacctggt gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac 
     4981 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 
     5041 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 
     5101 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 
     5161 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 
     5221 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 
     5281 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 
     5341 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 
     5401 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 
     5461 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 
     5521 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga 
     5581 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 
     5641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 
     5701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 
     5761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 
     5821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 
     5881 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 
     5941 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 
     6001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     6061 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 
     6121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     6181 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     6241 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 
     6301 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 
     6361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 
     6421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 
     6481 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     6541 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     6601 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 
     6661 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     6721 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     6781 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 
     6841 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 
     6901 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
     6961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
     7021 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
     7081 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
     7141 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
     7201 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
     7261 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 
     7321 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
     7381 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
     7441 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
     7501 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
     7561 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 
     7621 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
     7681 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
     7741 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
     7801 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     7861 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     7921 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     7981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     8221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 
     8821 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 
     8881 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 
     8941 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 
     9001 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 
     9061 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc 
     9121 tggagctgc 
//
